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D. Tucaliuc

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Articole semnate de acelasi autor in Revista Romana de Pediatrie:

Experimental evidence on the beneficial effect of early treatment with etanercept in juvenile idiophatic arthritis

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Revista Romana de PEDIATRIE | Volumul LXI, Nr. 1, An 2012
ISSN 1454-0398  |  e-ISSN 2069-6175
ISSN-L 1454-0398
DOI: 10.37897/RJP

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Experimental evidence on the beneficial effect of early treatment with etanercept in juvenile idiophatic arthritis

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ABSTRACT

Systemic inflammatory status is involved in early vascular lesions. Adjuvant-induced arthritis in rats is a commonly used experimental model of arthritis due to common features with rheumatoid arthritis in humans.

Aim: The aim of this study was to determine endothelio-dependent relaxation impairment generated by use of immunomodulatory therapy, etanercept.

Material and method: We used male, adult Wistar rats weighing 250-300 g. Arthritis was induced by a single intradermal injection of 0,1 ml complet Freund adjuvant into the plantar surface of the right hind-foot. Arthritis severity was quantified by pletismometry method. Etanercept therapy was performed twice a week, starting on day 22 post-adjuvant injection for 21 days. Vascular reactivity was performed on popliteal artery. After preconstriction with phenylephrine, we determined the vasodilatory response to acetylcholine.

Results: Acetylcholine-induced vasodilation in popliteal artery rings was significantly impaired in adjuvantinduced arthrits rats, compared to control rats. Etanercept reduces endothelial dysfunction in adjuvantinduced athritis rats.

Conclusions: Inflammatory status causes significant endothelial dysfunction in adjuvant-induced arthritis rats. Imunomodulatory therapy with etanercept significantly improves endothelial dysfunction.

Key words: endothelial dysfunction, adjuvant-induced arthritis, etanercept, rat

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DOVEZI EXPERIMENTALE PRIVIND EFECTUL BENEFIC AL TRATAMENTULUI PRECOCE CU ETANERCEPT IN ARTRITA JUVENILA IDIOPATICA

SELECT ISSUE

Revista Romana de PEDIATRIE | Volumul LXI, Nr. 1, An 2012
ISSN 1454-0398  |  e-ISSN 2069-6175
ISSN-L 1454-0398
DOI: 10.37897/RJP

Indexed

DOI - Crossref
Similarity Check by iThenticate, worldwide No 1 professional plagiarism checking system
DOAJ
Scopus
NLM Catalog
Ebsco Host - Medline
Semantic Scholar

HIGHLIGHTS

National Awards “Science and Research”

NEW! RJP has announced the annually National Award for "Science and Research" for the best scientific articles published throughout the year in the official journal.

ICMJE- Recommendations

Read the Recommendations for the Conduct, Reporting, Editing, and Publication of Scholarly work in Medical Journals.

Promoting Global Health

The published medical research literature is a global public good. Medical journal editors have a social responsibility to promote global health by publishing, whenever possible, research that furthers health worldwide.

DOVEZI EXPERIMENTALE PRIVIND EFECTUL BENEFIC AL TRATAMENTULUI PRECOCE CU ETANERCEPT IN ARTRITA JUVENILA IDIOPATICA

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REZUMAT

Statusul inflamator sistemic este implicat în leziunile vasculare incipiente. Artrita indusă de adjuvant la şobolan este un model de artrită experimentală frecvent utilizat, datorită caracteristicilor comune cu artrita reumatoidă.

Obiectiv: Studiu experimental privind afectarea relaxării endotelio-dependente la şobolani cu AIA şi efectul terapiei imunomodulatoare cu etanercept.

Material şi metodă: Am utilizat şobolani Wistar masculi, cu greutatea variind între 250-300 g. Artrita a fost indusă printr-o singură injectare intradermică de 0,1 ml adjuvant complet Freund la nivelul suprafeţei plantare a lăbuţei drepte. Severitatea artritei a fost cuantificată prin metoda pletismometrică. Injectarea subcutanată de etanercept s-a efectuat de două ori pe săptămână, începând cu ziua 22 postimunizare, timp de 21 de zile. Reactivitatea vasculară a fost determinată la nivelul arterei poplitee. După precontractarea cu fenilefrină, am determinat răspunsul vasodilatator la acetilcolină.

Rezultate: Vasodilataţia indusă de acetilcolină la nivelul inelelor de arteră poplitee este semnificativ mai mică în loturile de şobolani cu AIA, comparativ cu loturile de control. Etanerceptul reduce disfuncţia endotelială la şobolanii cu AIA.

Concluzii: Statusul inflamator cauzează o disfuncţie endotelială semnificativă la şobolanii cu AIA. Terapia imunomodulatoare cu etanercept a îmbunătăţit semnificativ disfuncţia endotelială.

Cuvinte cheie: disfuncţie endotelială, artită indusă de adjuvant, etanercept, şobolan

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